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P9827

Sigma-Aldrich

Polycytidylic acid–Agarose

lyophilized powder

Synonyme(s) :

Poly(C)–Agarose potassium salt

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About This Item

Numéro MDL:
MFCD00165464
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

lyophilized powder

Niveau de qualité

200

Ampleur du marquage

0.25-1.0 mg per mL

Matrice

cross-linked 4% beaded agarose

Activation de la matrice

cyanogen bromide

Fixation de matrice

amino

Espaceur de matrice

1 atom

Gonflement

1 g swells to ~5 mL

Température de stockage

−20°C

Application

Polycytidylic acid-agarose is used in protein chromatography, affinity chromatography and specialy resins. Polycytidylic acid has been used to study the preferential induction of apoptosis in the rainbow trout macrophage cell line. Polycytidylic acid has also been used to develop two analytical methods for human secretory-type ribonuclease.

Forme physique

Lyophilized powder stabilized with lactose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Preparation of sepharose-bound poly (rI:rC).
A F Wagner et al.
Biochemical and biophysical research communications, 45(1), 184-189 (1971-10-01)
C-P Benny Yiu et al.
Biochemical and biophysical research communications, 343(1), 311-318 (2006-03-18)
Dribble (DBE) is an essential protein in Drosophila that belongs to the evolutionarily conserved Krr1p protein family. Proteins in this family are localised in the cell nucleolus and are important for the processing of ribosomal RNAs. However, little is known
D Nadano et al.
Analytical biochemistry, 212(1), 111-116 (1993-07-01)
Two analytical methods for human secretory-type ribonuclease, which are based on polycytidylic acid/ethidium bromide fluorescence, have been developed. The first is a method for measurement of secretory-type ribonuclease activity utilizing the radial diffusion of ribonuclease in a thin agarose gel
S J Dewitte-Orr et al.
Fish & shellfish immunology, 18(4), 279-295 (2004-11-25)
The rainbow trout macrophage cell line RTS11 was found to be considerably more sensitive than rainbow trout fibroblast (RTG-2) and Chinook salmon epithelial (CHSE-214) cell lines to killing by macromolecular synthesis inhibitors, actinomycin D (AMD) and cycloheximide (CHX), a synthetic
Cristiana Valle et al.
Parasitology research, 96(4), 199-205 (2005-04-15)
We have cloned, sequenced and expressed a gene of Haemonchus contortus that encodes a protein (termed HcCYP) consisting of a cyclophilin domain and an RNA recognition motif (RRM). An antiserum raised against the recombinant protein showed that HcCYP was present
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