Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

P1588

Sigma-Aldrich

Placenta Growth Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

Synonyme(s) :

PGF

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD00677685
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Nom du produit

Placenta Growth Factor human, PlGF, recombinant, expressed in E. coli, powder, suitable for cell culture

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Essai

≥95% (SDS-PAGE)

Forme

powder

Qualité

endotoxin tested

Poids mol.

29 kDa

Conditionnement

pkg of 10 and 50 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

<1.0 EU/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Catégories apparentées

Actions biochimiques/physiologiques

Placenta growth factor (PlGF) is expressed in the placenta and somewhat less in the heart, lung and thyroid gland. Placentally expressed PlGF may act as an autocrine on trophoblasts, which express both PlGF and its receptor (VEGFR-1). Since these cells also make VEGF-A, natural heterodimers (PlGF/VEGF-A) have also been detected. Two alternatively spliced isoforms of PlGF have been identified.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Notes préparatoires

Recombinant human PlGF is lyophilized from a 0.2 micron filtered solution of 30% CH3CN and 0.1% TFA (pH 7.4) containing 50 microg of bovine serum albumin per 1 microg of cytokine.

Reconstitution

Reconstitute with sterile PBS containing at least 0.1% HSA or BSA. The rhP/GF concentration should be no less than 10 μg/mL.

Remarque sur l'analyse

The biological activity is measured by the ability to bind rhFlt-1/Fc in a functional ELISA. Immobilized rhFlt-1/Fc at 2 μg/mL (100 μL/well) can bind rhP/GF with a linear range at 0.1 - 5 ng/mL.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCK9291
SLCC6425
SLCC5139
SLCB6324
SLBV8157

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ai-Yi Zhou et al.
World journal of pediatrics : WJP, 10(3), 262-270 (2014-08-16)
Clinical trials have revealed that the antivascular endothelial growth factor (VEGF) therapies are effective in retinopathy of prematurity (ROP). But the low level of VEGF was necessary as a survival signal in healthy conditions, and endogenous placental growth factor (PIGF)
Domenico Ribatti
Angiogenesis, 11(3), 215-221 (2008-06-24)
The placental growth factor (PlGF) is an angiogenic protein belonging to the vascular endothelial growth factor (VEGF) family, which was discovered in 1991 by an Italian scientist, Maria Graziella Persico. Dr Persico cloned and purified PlGF and determined its structure
G Conn et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(4), 1323-1327 (1990-02-01)
A growth factor that is mitogenic for vascular endothelial cells, with an ED50 of approximately 1 ng/ml, has been purified 170,000-fold to apparent homogeneity from tissue culture medium conditioned by a rat glioma-derived cell line. The pure protein is a
Xu Zhou et al.
Scientific reports, 5, 10071-10071 (2015-05-12)
Cancer neovascularization plays an essential role in the metastasis of larynx carcinoma (LC). However, the underlying molecular mechanisms are not completely understood. Recently, we reported that placental growth factor (PLGF) regulates expression of matrix metalloproteinase 3 (MMP3) through ERK/MAPK signaling
Suk Ho Byeon et al.
Investigative ophthalmology & visual science, 51(2), 1190-1197 (2009-10-17)
Vascular endothelial cell growth factor (VEGF) is strongly induced by oxidative stress in retinal pigment epithelial (RPE) cells, and VEGF-A is a survival factor for various cell types. This study was conducted to determine whether the autocrine VEGF signaling pathway
Afficher tous les articles scientifiques apparentés

Articles

Vascular Endothelial Growth Factors (VEGF)

VEGF-A stimulates blood vessel growth via endothelial cell proliferation, migration, and survival during embryonic development.

Vascular Endothelial Growth Factors (VEGF)

VEGF-A stimulates blood vessel growth via endothelial cell proliferation, migration, and survival during embryonic development.

Vascular Endothelial Growth Factors (VEGF)

VEGF-A stimulates blood vessel growth via endothelial cell proliferation, migration, and survival during embryonic development.

Vascular Endothelial Growth Factors (VEGF)

VEGF-A stimulates blood vessel growth via endothelial cell proliferation, migration, and survival during embryonic development.

Afficher tout

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique