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N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Synonyme(s) :

4-Nitrophenyl phosphate disodium salt solution, pNPP

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About This Item

Formule linéaire :
O2NC6H4OP(O)(ONa)2
Numéro CAS:
4264-83-9
Poids moléculaire :
263.05
Numéro MDL:
MFCD00007319
Code UNSPSC :
12352204
ID de substance PubChem :
24897835
Nomenclature NACRES :
NA.83

product name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

Forme

liquid

Conditions d'expédition

wet ice

Température de stockage

−20°C

Chaîne SMILES 

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

Clé InChI

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description générale

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Actions biochimiques/physiologiques

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Attention

Stable at least one year when stored below 0 °C.

Forme physique

Ready-to-use.

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H290,H315,H318,H361fd,H373

Classification des risques

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Organes cibles

Kidney,Liver,Blood

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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European journal of medicinal chemistry, 178, 108-115 (2019-06-09)
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Cheng-Chi Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(33), 11661-11666 (2008-08-12)
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Humanization of anti-human insulin receptor antibody for drug targeting across the human blood-brain barrier
Boado RJ, et al.
Biotechnology and Bioengineering, 96(2), 381-391 (2007)
Peter Van den Eede et al.
PloS one, 6(1), e16257-e16257 (2011-02-08)
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International journal of biomedical research, 80(8), 467-474 (2007)
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