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MAK562

Sigma-Aldrich

Branched Chain Amino Acid Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

BCAA Assay Kit, BCAA Kit

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About This Item

Code UNSPSC :
41116104
Nomenclature NACRES :
NA.28

Utilisation

sufficient for 100 colorimetric tests

Niveau de qualité

100

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; neurological disorders; gastrointestinal diseases

Température de stockage

−20°C

Description générale

Leucine, isoleucine and valine, known collectively as branched-chain amino acids (BCAA) are unique amongst the essential amino acids in that they undergo oxidation to a greater extent in the peripheral tissues than in liver1. Concentrations of these amino acids in plasma are altered by moderate and exhausting exercise, by diet and the nutritional status of the individual.2

In this assay, BCAA concentration is determined using a coupled enzyme reaction to catalyze the conversion of a BCAA molecule to its ketone form, which results in the conversion of NAD+ to NADH.3 NADH is detected by a colorimetric indicator, with an absorption maximum of 450 nm, proportional to the amount of BCAA present.

Application

BCAA Kit has been used to quantify BCAA in various types of samples, such as food, dietary supplements, blood, serum and cells.

Caractéristiques et avantages

Highly sensitive, simple and high-throughput assay for measuring BCAA concentration.
Detection time: only 30 minutes
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Adéquation

Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological and organic samples.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314

Classification des risques

Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Enzymic determination of branched-chain amino acids and 2-oxoacids in rat tissues. Transfer of 2-oxoacids from skeletal muscle to liver in vivo
Livesey G, et al.
The Biochemical Journal, 188, 705-713 (1980)
P W Lemon et al.
Medicine and science in sports and exercise, 13(3), 141-149 (1981-01-01)
At rest, especially during starvation, protein (via hepatic gluconeogenesis and/or direct oxidation) contributes significantly to the body's total metabolic requirement. During exercise, carbohydrate (CHO) and free fatty acids (FFA) are generally regarded as the major energy sources. Although protein has
A simple enzymatic fluorimetric method for the determination of branched-chain L-amino acids in microlitre volumes of plasma
Gleeson M, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 166, 163-169 (1987)

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