The volume of this product will vary from lot to lot. The minimum concentration is 5 mg/mL, however a survey of recent lots shows an average of 13 mg/mL. A 1mg vial will have an average volume of 77 uL. The exact protein concentration is reported on the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l9420#product-documentation
L9420
Luciferase from Photinus pyralis (firefly)
recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
Synonyme(s) :
Luciferase firefly
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Essai
≥98% (SDS-PAGE)
Forme
buffered aqueous solution
Activité spécifique
≥10×1010 units/mg protein
Poids mol.
62 kDa
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light.[1] The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.[2]
Application
Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.[3]
Définition de l'unité
One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Forme physique
Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral
Organes cibles
Kidney
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
231.8 °F
Point d'éclair (°C)
111 °C
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Certificats d'analyse (COA)
Lot/Batch Number
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Les clients ont également consulté
Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Isabella Nymann Westensee et al.
Small (Weinheim an der Bergstrasse, Germany), 17(24), e2007959-e2007959 (2021-05-11)
Artificial cells (ACs) aim to mimic selected structural and functional features of mammalian cells. In this context, energy generation is an important challenge to be addressed when self-sustained systems are desired. Here, mitochondria isolated from HepG2 cells are employed as
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Tanushree Dangi et al.
Cell reports, 42(3), 112167-112167 (2023-03-02)
mRNA vaccines are effective in preventing severe COVID-19, but breakthrough infections, emerging variants, and waning immunity warrant the use of boosters. Although mRNA boosters are being implemented, the extent to which pre-existing immunity influences the efficacy of boosters remains unclear.
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the Karrikin signaling pathway.
Sun Y K, et al.
Frontiers in Plant Science, 7, 1799-1799 (2016)
Articles
Bioluminescence imaging (BLI) systems allows for high-sensitive and noninvasive monitoring of cell proliferation, activity of signaling pathways and protein-protein interactions in living tissues.
seMpai substrate is suitable for near-infrared BLI in biological experiments, offering high solubility for extended bioluminescent applications.
seMpai substrate is suitable for near-infrared BLI in biological experiments, offering high solubility for extended bioluminescent applications.
seMpai substrate is suitable for near-infrared BLI in biological experiments, offering high solubility for extended bioluminescent applications.
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