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HYSHP020

Sigma-Aldrich

HyStem®-HP Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution

Synonyme(s) :

Cell Culture Scaffold, HP Cell Culture Kit, HyStem-HP Scaffold Kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

100

Température de stockage

−20°C

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Catégories apparentées

Description générale

The HyStem®-HP Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture, where slowly released growth factors are crucial in re-creating a stem cell niche. To affect specific cell performance, growth factors or ECM proteins may be added to the HyStem-HP hydrogel. Unlike animal-derived ECM products, this kit contains three fully chemically defined components, which are nonimmunogenic:
  • HyStem®-HP – a thiol-modified hyaluronan (a major constituent of native ECM), carboxymethyl hyaluronic acid-thiopropanoyl hydrazide (CMHA-S, CMHA-DTPH, carboxymethyl hyaluronic acid-DTPH) with heparinthiopropanoyl hydrazide (HP-DTPH). HyStem-HP (CMHA-DTPH with HP-DTPH) contains 99.7 wt% CMHA-DTPH and 0.3 wt% HP-DTPH.
  • Gelin-S™ – a thiol-modified gelatin (denatured collagen), carboxymethyl gelatin-thiopropanoyl hydrazide (GTN-DTPH, carboxymethyl gelatin-DTPH)
  • Extralink® – a thiol-reactive crosslinker, polyethylene glycol diacrylate (MW = 3,400 g/mole, PEGDA)

Caractéristiques et avantages

Customizable, well characterized and consistent, Hystem-HP hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-HP offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-HP offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-HP contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), Heparin and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-HP hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.
Hystem-HP enables ionic binding and slow release of growth factors through thiolated heparin; growth factors can be integrated via the matrix rather than cell culture medium. Hystem-HP also enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-HP, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-HP is recommended for both in vivo and in vitro experimentation.

Informations légales

Extralink is a registered trademark of Glycosan BioSystems, Inc.
Gelin is a trademark of Biopharm Ltd.
Hystem is a registered trademark of BIOTIME, INC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

CorrosionExclamation mark
GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H317,H318

Classification des risques

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Guangyue Li et al.
Oxidative medicine and cellular longevity, 2022, 3319056-3319056 (2022-10-21)
Advanced glycation end products (AGEs) accumulate in the bone tissue of patients with diabetes mellitus, resulting in oxidative stress, poor bone healing, or regeneration. Irisin, a novel exercise-induced myokine, is involved in the regulation of bone metabolism. However, the effects

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