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GE28-9031-32

Protein A High Performance Spintrap

Cytiva 28-9031-32, pack of 16 columns

Synonyme(s) :

High Performance Spintrap, Protein A Spintrap

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.25

ligand

native protein A

Caractéristiques

wetted part: no

Conditionnement

pack of 16 columns

Fabricant/nom de marque

Cytiva 28-9031-32

Conditions de stockage

(20% Ethanol)

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning

2-10

working range

3-9

Capacité

>1 mg binding capacity(human IgG well)

Température de stockage

2-8°C

Description générale

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein A HP SpinTrap are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose HP.

Application

Protein A Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Caractéristiques et avantages

  • For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provide high yield.
  • Prepacked with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein A HP prepacked columns.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Protocoles

This page shows how to separate IgG antibodies by affinity chromatography using Protein A HP SpinTrap columns from Cytiva.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

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