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Key Documents

GE17-6003-18

DeStreak Reagent

Cytiva 17-6003-18

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About This Item

Numéro CE :
Code UNSPSC :
41105323
Nomenclature NACRES :
NA.25

Forme

liquid

Caractéristiques

wetted part: no

Conditionnement

pkg of 1 mL

Fabricant/nom de marque

Cytiva 17-6003-18

Conditions de stockage

(Rehydration Solution -20)

Couleur

Colorless

Température de stockage

2-8°C

Description générale

DeStreak Reagent.

Application

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.

DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

DeStreak Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak Reagent is also available separately in a 1-ml pack size.

Caractéristiques et avantages

  • Improves reproducibility and quality of 2-D gels by preventing streaking.
  • Eliminates extra spots caused by nonspecific oxidation of Proteins.
  • Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
  • Highly suitable for both analytical and preparative gels.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

DeStreak is a trademark of Cytiva

Pictogrammes

Skull and crossbones

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Acute Tox. 3 Oral - Eye Dam. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

233.6 °F - closed cup

Point d'éclair (°C)

112 °C - closed cup


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Protocoles

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

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