GE17-0948-01
Blue Sepharose™ 6 Fast Flow
Cytiva 17-0948-01, pack of 50 mL
Synonyme(s) :
Blue Sepharose Fast Flow
About This Item
Produits recommandés
ligand
Cibacron Blue
Conditionnement
pack of 50 mL
Fabricant/nom de marque
Cytiva 17-0948-01
Conditions de stockage
(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
Matrice
6% cross-linked agarose
Taille des particules
45-165 μm
Diamètre moyen
90 μm
cleaning in place
3-13
working range
4-12
Adéquation
suitable for bioprocess medium
Température de stockage
2-8°C
Description générale
Blue Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
Blue Sepharose™ 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production.
Blue Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Blue Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Caractéristiques et avantages
- Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
- BioProcess medium supported for industrial applications and well-established in approved processes
- Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
Protocoles
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.
Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.
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