Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

GE17-0920-07

IMAC Sepharose High Performance

Cytiva 17-0920-07, pack of 100 mL

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Conditionnement

pack of 100 mL

Fabricant/nom de marque

Cytiva 17-0920-07

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

working range

3-12

Capacité

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Description générale

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Application

Conveniently charge with your metal of choice. IMAC Sepharose FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.

Caractéristiques et avantages

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
  • Conveniently charge with your metal of choice
  • 34 μm bead size for high performance purification
  • High binding capacity

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

Afficher tout

Protocoles

Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Afficher tout

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique