Gamborg's B-5 Basal Medium with Minimal Organics is a plant tissue culture medium that contains reduced amounts of organic compounds compared to the standard Gamborg's B-5 Basal Medium. The minimal organics version is designed to provide a lower concentration of organic compounds, such as vitamins or amino acids, which allows for more precise control over nutrient supplementation in plant tissue culture experiments. Please see the link below to review the formulation tables for Gamborg's basal salt mixtures, products G5893 and G5768.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/186/351/g5768for.pdf
G5893
Gamborg′s B-5 Basal Medium with Minimal Organics
Gamborg’s B-5 Basal Medium with Minimal Organics
fine powder, suitable for plant cell culture
Synonyme(s) :
B-5 Basal Medium, Basal Medium, Minimal Organics Medium
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.72
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Forme
fine powder
Niveau de qualité
Technique(s)
cell culture | plant: suitable
Application(s)
agriculture
Température de stockage
2-8°C
Description générale
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.[1]
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Application
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
- as a component of liquid media for the tissue culture of Brassica rapa[2]
- as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings[3]
- as a component of solid GM medium for germination of Arabidopsis seeds[4]
Variante de formule
With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
Quantité
Formulated to contain 3.2 grams of powder per liter of medium.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Ox. Sol. 3
Code de la classe de stockage
5.1B - Oxidizing hazardous materials
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Experiments in Plant Tissue Culture (1985)
Jakob Petereit et al.
Frontiers in plant science, 8, 72-72 (2017-02-24)
In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown
Martin E Kolewe et al.
Plant cell reports, 29(5), 485-494 (2010-03-11)
Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus
Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion in the Culture Medium
Habitation (Elmsford, N.Y.), 153-173 (2020)
RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
Lackey E, et al.
The New phytologist, 186(1), 207-215 (2010)
Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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