F4274

Fluorescein isothiocyanate isomer I

≥97.5% purity (HPLC), powder

• Synonyme(s) : FITC, Fluorescein 5-isothiocyanate
• Formule empirique (notation de Hill) : C₂₁H₁₁NO₅S
• Numéro CAS: 3326-32-7
• Poids moléculaire : 389.38
• NACRES: NA.47
• PubChem Substance ID: 24894860
• UNSPSC Code: 12171500
• EC Number: 222-042-0
• MDL number: MFCD00005063
• Beilstein/REAXYS Number: 1407295

Propriétés

• Nom du produit: Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
• assay: ≥97.5% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to very dark yellow
• mp: >360 °C (lit.)
• solubility: acetone: soluble 10 mg/mL
• fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Réactif pour le marquage FITC des protéines; le microséqueçage de protéines et de peptides (HPLC)

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Informations sur la sécurité

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves