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F2266

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

sheep

Niveau de qualité

Conjugué

FITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct immunofluorescence: 1:128

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

IgGs are glycoprotein antibodies that regulate immunological defense mechanisms such as phagocytosis and inflammatory responses . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibodies are specific for mouse IgG subclasses G1, G2a, G2b, and G3 and do not react with serum proteins in rat.

Immunogène

Mouse IgG

Application

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody is suitable for use in flow cytometry, immunohistochemistry, indirect immunofluorescence, and immunohistochemistry .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Autres remarques

Antibody adsorbed with rat serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Notes préparatoires

Adsorbed to reduce background staining with rat samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Y Yan et al.
Journal of immunology (Baltimore, Md. : 1950), 166(8), 5258-5264 (2001-04-06)
Donor leukocytes play a dual role in rejection and acceptance of transplanted organs. They provide the major stimulus for rejection, and their removal from the transplanted organ prolongs its survival. Paradoxically, administration of donor leukocytes also prolongs allograft survival provided
A Mechaly et al.
Applied and environmental microbiology, 74(3), 818-822 (2007-10-30)
A single-chain Fv (scFv) antibody was developed and applied for efficient and specific detection of Bacillus anthracis spores. The antibody was isolated from a phage display library prepared from spleens of mice immunized with a water-soluble extract of the outer
C M Newman et al.
Arteriosclerosis, thrombosis, and vascular biology, 15(11), 2010-2018 (1995-11-01)
Osteopontin (OP) is a secreted glycoprotein that contains the Arg-Gly-Asp (RGD) cell-binding sequence that binds calcium and is chemotactic and adhesive for rat vascular smooth muscle cells (VSMCs). OP gene expression is upregulated in cultured rat VSMCs in vitro and
Sambit K Nanda et al.
The Journal of experimental medicine, 208(6), 1215-1228 (2011-05-25)
The protein ABIN1 possesses a polyubiquitin-binding domain homologous to that present in nuclear factor κB (NF-κB) essential modulator (NEMO), a component of the inhibitor of NF-κB (IκB) kinase (IKK) complex. To address the physiological significance of polyubiquitin binding, we generated
Hisashi Ueta et al.
Hepatology (Baltimore, Md.), 47(4), 1352-1362 (2008-01-29)
Donor dendritic cell (DC) migration and allosensitization in host secondary lymphoid organs after liver transplantation are ill defined. We used rat models to investigate graft-derived cells and intrahost allosensitization. Liver transplantation induced diffuse blood-borne migration of donor major histocompatibility class

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