Yes. stock solutions in ethanol may be sterile filtered. However, cellulose-based filter membranes are not recommended for use with ethanol. Please see the link below to review additional information regarding filter membrane compatibility with commonly used solvents:
https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/chemical-compatibility-of-filter-components
E2758
β-Estradiol
BioReagent, powder, suitable for cell culture
Synonyme(s) :
1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
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About This Item
Formule empirique (notation de Hill):
C18H24O2
Numéro CAS:
Poids moléculaire :
272.38
Beilstein:
1914275
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352209
ID de substance PubChem :
Nomenclature NACRES :
NA.77
Source biologique:
synthetic (organic)
Forme:
powder
Essai:
≥98% (HPLC)
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
synthetic (organic)
Niveau de qualité
Gamme de produits
BioReagent
Essai
≥98% (HPLC)
Forme
powder
Technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
Pf
176-180 °C (lit.)
Solubilité
ethanol: 50 mg/mL, clear, colorless
Conditions d'expédition
ambient
Température de stockage
room temp
Chaîne SMILES
O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
InChI
1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
Clé InChI
VOXZDWNPVJITMN-ZBRFXRBCSA-N
Informations sur le gène
human ... ESR1(2099) , ESR2(2100) , ESRRA(2101) , ESRRB(2103) , GPER(2852) , SERPINA6(866)
mouse ... Esr1(13982) , Esr2(13983) , Esrra(26379)
rat ... Afp(24177) , Ar(24208) , Esr1(24890) , Esr2(25149) , Shbg(24775)
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Description générale
β-Estradiol or estradiol-17 β (E2) is secreted majorly by the large preovulatory follicles in the ovary. In addition, estradiol can be synthesized de novo from cholesterol have androgens synthesized from cholesterol. The aromatization of androgen by enzyme aromatase (P450arom) results in the β-estradiol production.[1]
Application
β-Estradiol has been used:
- as a component of Dulbecco′s modified Eagle medium (DMEM) for culturing mammary tumor cells[2]
- as a oestrogenic compound for testing neuroprotective effects[3]
- as a component of medium199 for culturing oocytes[4]
β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).
Actions biochimiques/physiologiques
The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.
β-Estradiol (E2) is a major premenopausal hormone in women and is essential for spermiogenesis, sperm maturation and motility in men.[5] Cultured bone marrow stem cells (BMSCs) have ability to synthesize E2 and could be exploited for treating estrogen deficiency.[1] It mediates physiological functions through estrogen receptor (ER) α and ERβ.[6]
The major estrogen secreted by the premenopausal ovary.
Forme physique
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Reconstitution
To prepare a 20 μg/ml stock solution, add 1 ml absolute ethanol to 1 mg β-estradiol; gently swirl to dissolve; add 49 ml sterile medium while mixing.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Certificats d'analyse (COA)
Lot/Batch Number
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Les clients ont également consulté
Selective oestrogen receptor (ER) modulators reduce microglia reactivity in vivo after peripheral inflammation: potential role of microglial ERs
Tapia-Gonzalez S, et al.
The Journal of Endocrinology, 198, 219?230-219?230 (2008)
Estrogen synthesis and signaling pathways during aging: from periphery to brain
Cui J, et al.
Trends in Molecular Medicine, 19(3), 197-209 (2013)
The developmental potential of oocytes is impaired in cattle with liver abnormalities
Sarentonglaga B, et al.
Journal of Reproduction and Development, 59(2), 168-173 (2013)
Estradiol synthesis and release in cultured female rat bone marrow stem cells
Zhang D, et al.
BioMed Research International, 2013 (2013)
Loss of STAT1 from mouse mammary epithelium results in an increased Neu-induced tumor burden
Klover PJ, et al.
Neoplasia, 12(11), 899-905 (2010)
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Can Estradiol/ethanol solutions be sterile filtered?
1 answer-
Helpful?
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Hello, I would like to check the solubility of this product in DMSO. Best,
1 answer-
The solubility of this product in DMSO has not been determined. However, as per the literature, the β-Estradiol is soluble in DMSO at 20 mg/ml.
Helpful?
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after dissolving the β-Estradiol in ethanol , whats the tempreture to store the item in ?
1 answer-
The storage or stability conditions once the compound is in solution is not determined. General recommendations for β-Estradiol are to protect from light, and store at -20C or at 2-8C in a degassed solution. Solutions stored in this manner should remain stable in the range of 1 month - 1 year.
Helpful?
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How diluit for concentration of the 100ng/mL
1 answer-
β-Estradiol is soluble in absolute ethanol at 50 mg/mL (See E8875). Preparing a 100 ng/mL solution would require serial dilutions.
Start with a 1 mg/mL (1000 ug/mL) stock in ethanol.
Dilute 10 uL (10 ug = 10,000 ng) of this stock with 9.990 mL to achieve a 1000 ng/mL solution. This can be diluted with medium or buffer.
Dilute 1 mL of this with 9 mL to the final concentration of 100 ng/mL.Helpful?
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