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D9281

Sigma-Aldrich

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

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About This Item

Code UNSPSC :
41141827
Nomenclature NACRES :
NA.25

Forme

liquid

Utilisation

100 uses (0.1 mg size)
500 uses (0.5 mg size)

Adéquation

suitable for electrophoresis (DNA)

Température de stockage

−20°C

Description générale

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Composants

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Autres remarques

Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.

Notes préparatoires

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

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Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

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Protocoles

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

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GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

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