D7385

Demecolcine

≥98% (HPLC)

• Synonyme(s) : N-Deacetyl-N-methylcolchicine
• Formule empirique (notation de Hill) : C₂₁H₂₅NO₅
• Numéro CAS: 477-30-5
• Poids moléculaire : 371.43
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24894128
• EC Number: 207-514-6
• Beilstein/REAXYS Number: 2822892
• MDL number: MFCD00075459

Propriétés

• Quality Segment: 200
• assay: ≥98% (HPLC)
• form: powder
• mp: 184 °C
• solubility: chloroform: 10 mg/mL, ethanol: soluble
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
• InChI key: NNJPGOLRFBJNIW-HNNXBMFYSA-N

Description

Application

Demecolcine has been used as a microtubule depolymerizing agent. It has also been used to inhibit mitotic division.

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G₁ and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Informations sur la sécurité

• pictograms: GHS06
• signalword: Danger
• hcodes: H300
• pcodes: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges