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CS1000

Sigma-Aldrich

Superoxide Anion Assay Kit

sufficient for 100 assays (96 well plates)

Synonyme(s) :

Superoxide Detection Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 assays (96 well plates)

Niveau de qualité

200

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Application

The Superoxide Anion assay kit provides a useful qualitative cell-based assay for the measurement of O2− anion status in cells. The kit can be used to test changes in superoxide anion levels following oxidative stress directly in whole cells. In addition, the kit can be also used for the detection of superoxide dismutase (SOD) activity. This activity was tested on erythrocytes, leucocytes, and plasma.

Actions biochimiques/physiologiques

The superoxide anion (O2-) is a short-lived radical generated by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and γ-radiation, or by oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components causing damage to lipids, proteins, and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.

Remarque sur l'analyse

The kit method is based on the oxidation of luminol by superoxide anions resulting in the formation of chemiluminescence light. This method utilizes a specific, non-toxic enhancer that amplifies the chemiluminescent signal.

Composants de kit seuls

Réf. du produit
Description
  • Assay Buffer 25 mL
  • Assay Medium 20 mL
  • Enhancer Solution, 40 mM 500 μL
  • Luminol Solution 500 μL
  • Xanthine Solution 500 μL
  • Xanthine Oxidase .5 U
  • Superoxide Dismutase from bovine liver, 4 units/μL 400 U

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Muthusamy V Shanmuganathan et al.
Microbes and infection, 16(2), 134-141 (2013-10-29)
Macrophages serve as permissive niches for Escherichia coli (E. coli) K1 to attain high grade bacteremia in the pathogenesis of meningitis in neonates. Although pterin levels are a diagnostic marker for immune activation, the role of macrophages in pterin production
Wen-Bin Zhang et al.
Oxidative medicine and cellular longevity, 2021, 6610543-6610543 (2021-02-06)
Doxorubicin- (DOX-) related cardiac injury impairs the life quality of patients with cancer. This largely limited the clinical use of DOX. It is of great significance to find a novel strategy to reduce DOX-related cardiac injury. Oroxylin A (OA) has
Dawei Jiang et al.
Nature biomedical engineering, 2(11), 865-877 (2018-12-07)
Patients with acute kidney injury (AKI) frequently require kidney transplantation and supportive therapies, such as rehydration and dialysis. Here, we show that radiolabelled DNA origami nanostructures (DONs) with rectangular, triangular and tubular shapes accumulate preferentially in the kidneys of healthy
Erythropoietin activates nitric oxide synthase in murine erythrocytes
Mihov,D et al.
The American Journal of Physiology, 279(2), C378-C388 (2009)
Kyoung Ah Kang et al.
Redox biology, 37, 101698-101698 (2020-08-31)
We have previously shown that non-thermal dielectric-barrier discharge (DBD) plasma induces the generation of reactive oxygen species (ROS) in cells; however, the underlying mechanism has not been elucidated. This study aimed to identify the mechanisms through which DBD plasma induces
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