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CS0980
Chitinase Assay Kit
sufficient for 100 multiwell tests
Synonyme(s) :
Chitinase Activity Detection Kit
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| Conditionnement | Référence | Disponibilité | Prix |
|---|
A propos de cet article
usage
sufficient for 100 multiwell tests
Quality Level
absorbance ratio
405 nm (Absorbance)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... CHIA(27159), CHIT1(1118)
Catégories apparentées
1 of 1
Cet article | |||
|---|---|---|---|
| Gene Information human ... CHIA(27159), CHIT1(1118) | Gene Information human ... CHIT1(1118) | Gene Information human ... GSR(2936) | Gene Information human ... CS(1431) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
| usage sufficient for 100 multiwell tests | usage sufficient for 200 multiwell tests | usage sufficient for 700 assays | usage kit sufficient for 100 reactions (using a 1 ml cuvette), kit sufficient for 480 reactions (using 96 multiwell plates) |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| absorbance ratio 405 nm (Absorbance) | absorbance ratio - | absorbance ratio - | absorbance ratio - |
General description
Application
The kit provides all the reagents required for efficient detection of chitinase activity in fungal and bacterial growth media, macrophage lysates, and purified enzyme preparations. In addition, the kit provides three different substrates for the detection of the various types of the chitinolytic activity:
- 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside - substrate suitable for exochitinase activity detection (chitobiosidase activity)
- 4-Nitrophenyl N-acetyl-β-D-glucosaminide - substrate suitable for exochitinase activity detection (β-N-acetylglucosaminidase activity)
Biochem/physiol Actions
The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase, respectively.
Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack.
Analysis Note
Composants de kit seuls
- Assay Buffer 20 mL
- 4-Nitrophenyl N-acetyl-β-D-glucosaminide 10 mg
- 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside 5 mg
- 4-Nitrophenyl β-D-N,N′N′′-triacetylchitotriose 1 mg
- Chitinase from Trichoderma viride 1 mg
- p-Nitrophenol Solution, 10 mM 1 mL
- Sodium Carbonate 1 g
related product
signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Resp. Sens. 1 - STOT RE 2 Oral
target_organs
Liver,Kidney
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
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Contenu apparenté
Instructions
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| CS0980-1KT | 04061833553169 |
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Hello, I purchasee chitinase assay kit CS0980 last month. Can I use the kiy to analyse chitinase in hepatopancreas of lobsters? I dont find the procedure in the kit to prepare and measure chitinase from tissue samples
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As noted in its Technical Bulletin, the kit has been tested on Trichoderma viride, Streptomyces griseus and human macrophages. No protocol is available for the use of the kit to determine Chitinase activity in hepatopancreas of lobsters.
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We are looking for established CHIT1enzyme activity assays for analysis of clinical samples (human serum or plasma).Does this kit apply to that?
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Yes, this kit is suitable for analyzing human serum and plasma. Please note that this product is for R&D use only, not for drug, household, or other uses.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do you make the substrate in Product CS0980, Chitinase Assay Kit, go into solution?
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The substrates do not dissolve easily in the buffer. It may take ~1 hour of shaking to completely dissolve the substrates. Use of a larger container (15 mL) may aid dissolution.
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What is the recommended substrate concentration for Product CS0980, Chitinase Assay Kit?
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A 1mg/mL substrate solution is recommended. If required, the 4-Nitrophenyl-beta-D-N,N',N''-triacetylchitotriose and the 4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside can be used at lower concentrations in the reaction (0.5 or 0.2mg/mL respectively).
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What is the stability of the Product CS0980, Chitinase Assay Kit, substrate solution (s)?
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The substrate solution(s) should be stored on ice during the experiment. For storage up to one month, freeze at -20°C or below.
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How many reactions will each substrate in Product CS0980, Chitinase Assay Kit, provide?
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If used at a concentration of 1mg/mL: * 4-Nitrophenyl N-acetyl-b-D-glucosaminide (Product No. N9376) -100 reactions; * 4-Nitrophenyl N,N’-diacetyl-b-D-chitobioside (Product No. N6133) -50 reactions; * 4-Nitrophenyl-b-D-N,N',N''-triacetylchitotriose (Product No. N8638) -10 reactions.
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What has Product CS0980, Chitinase Assay Kit, been tested on?
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This kit has been tested on Trichoderma viride, Streptomyces griseus and human macrophages.
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