CHP2
Imprint® Ultra Chromatin Immunoprecipitation Kit
Complete ChIP kit for sensitivity, compatibility with Next-Gen sequencing
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352202
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Description générale
The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.
Application
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Caractéristiques et avantages
- Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
- Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
- Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
- Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Informations légales
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit seuls
Réf. du produit
Description
- Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
- Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- S6576ChIP Next Gen Seq Sepharose™FDS
- I8896IGEPAL® CA-630, for molecular biologyFDS
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureFDS
- W4502Water, Nuclease-Free Water, for Molecular BiologyFDS
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderFDS
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderFDS
- A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)FDS
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)FDS
- D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridizationFDS
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionFDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionFDS
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)FDS
Afficher tout (12)
En option
Réf. du produit
Description
Tarif
Produit(s) apparenté(s)
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3
Organes cibles
Central nervous system
Code de la classe de stockage
5.1A - Strongly oxidizing hazardous materials
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
Si vous avez besoin d'assistance, veuillez contacter Service Clients
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
Yoon-A Kang et al.
Molecular and cellular biology, 32(1), 226-239 (2011-10-26)
Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery functions in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy
Protocoles
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
