NCI-H292

91091815, human lung, Epithelial

• Synonyme(s) : H292 Cells, NCI-HUT-292 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: NCI-H292, 91091815
• Source biologique: human lung
• Mode de croissance: Adherent
• Caryotype: Modal no. 47
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human lung, mucoepidermoid carcinoma

Description de la lignée cellulaire

Derived from a cervical node metastasis of a pulmonary mucoepidermoid carcinoma from a 32 year old female. Cells retain mucoepidermoid characteristics in culture and support replication of Hepatitis B virus. Some of the cells contain numerous small mucin-containing granules and are L-DOPA decaroxylase negative. This cell line has been selected as a prototype for transfecting subgenome fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells are mucicarmine positive and also stain positively for keratin and vimentin but negative for neurofilament triplet protein. Ethnicity: Black.

Application

NCI-H292 has been used to determine the ability of the cell line to support the replication of mumps virus.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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