SK-OV-3 Cell Line human

91091004, human ovary, Epithelial

• Synonyme(s) : SKOV-3 Cells, SKOV3 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: SK-OV-3 Cell Line human, 91091004
• Source biologique: human ovary
• Description: Human Caucasian ovary adenocarcinoma
• Mode de croissance: Adherent
• Caryotype: Hypodiploid to hypotetraploid
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian ovary adenocarcinoma

Description de la lignée cellulaire

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Application

SK-OV-3 cell line from humans has been used to study chimeric adenoviruses of Ad5 (species C) displaying proteins of the naturally enteric Ad40 (species F) as vectors for gene delivery.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

Milieu de culture

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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