AK-D

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89071903, feline lung, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Source biologique: feline lung
• Conditionnement: tube of 5 μg 89071903-DNA-5UG; pkg of vial of cells 89071903-1VL
• Mode de croissance: Adherent
• Caryotype: Diploid
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Foetal cat lung normal

Description de la lignée cellulaire

A clonal derivative of mass cultures of foetal feline lung after 14 passages in vitro. Susceptible to panleukopenia, feline herpes and feline picornaviruses.

Application

Virus studies: panleucopenia, feline herpes and feline picornaviruses

Milieu de culture

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin and 0.03% EDTA; 5% CO₂; 37°C.

Autres remarques

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