Grunt Fin (GF)

88010601, fish fin, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• product name: Grunt Fin (GF), 88010601
• Source biologique: fish fin
• Mode de croissance: Adherent
• Caryotype: Modal no. 47
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Fish Grunt fin tissue blue striped

Description de la lignée cellulaire

Derived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.

Milieu de culture

EMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO₃ + 20% FBS (Heat Inactivated)+ 58mM NaCl

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low a

Autres remarques

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