MG-63 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86051601, human bone, Fibroblast

• Synonyme(s) : MG63 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human bone
• Description: Human osteosarcoma
• Conditionnement: tube of 5 μg 86051601-DNA-5UG; pkg of vial of cells 86051601-1VL
• Mode de croissance: Adherent
• Caryotype: Modal no. 66; hypotriploid
• Morphologie: Fibroblast
• Produits: Inducible interferon
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human osteosarcoma

Description de la lignée cellulaire

From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.

Application

Human MG-63 cell line has been used:

• to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells
• to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion and proliferation of MG-63 cells
• as a positive control for immunostaining procedure
• to study the interactions and effects of lysophosphatidate functionalized titanium on MG-63 cells

Interferon-induction studies

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.