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CHO-K1 Cell Line from hamster

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85051005, hamster ovary, Epithelial

Synonyme(s) :

CHO cell clone K1 Cells, CHOK1 Cells

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About This Item

Code UNSPSC :
41106514
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

hamster ovary

Conditionnement

tube of 5 μg 85051005-DNA-5UG
pkg of vial of cells 85051005-1VL

Mode de croissance

Adherent

Caryotype

Hypodiploid, modal no. 20

Morphologie

Epithelial

Produits

Not specified

Récepteurs

Not specified

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

dry ice

Température de stockage

−196°C

Catégories apparentées

Origine de la lignée cellulaire

Hamster Chinese ovary

Description de la lignée cellulaire

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

Application

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.[1][2]
Nutritional and gene expression studies

Milieu de culture

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25%Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

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Cell Lines for Recombinant Protein Expression

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.

Questions

1–9 of 9 Questions  

  1. What is the approximate cells/cm2 of a 100% Confluent flask?

    1 answer

    1. A general guideline for the approximate number of cells per square centimeter in a 100% confluent flask is 1 x 10^5 cells/cm². For more detailed information, refer to the table on page 4 of the following brochure: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/318/066/corning_cc_guide.pdf

      Helpful?

  2. Is the cell like deficient of DHFR gene?

    1 answer

    1. CHO-K1 are dihydrofolate reductase (DHFR) positive. There are other mutants made from CHO-K1 that are DHFR deficient. Please see the links below to review these possible options:

      CHO/dhFr-
      https://www.sigmaaldrich.com/product/sigma/cb_94060607

      CHO/dhFr-AC-free
      https://www.sigmaaldrich.com/product/sigma/cb_05011002

      CHOZN® ZFN-Modified CHO
      https://www.sigmaaldrich.com/product/sigma/chodhfr

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  3. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  4. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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  5. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  6. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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  7. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  8. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

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  9. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

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