MRC-5 PD 19 Cell Line human

5072101, human lung, Fibroblast-like

• Synonyme(s) : MRC-V Cells, MRC5 Cells, Medical Research Council Cell Strain-5
• Code UNSPSC : 41106514

Propriétés

• product name: MRC-5 PD 19 Cell Line human, 05072101
• Source biologique: human lung
• Description: Human fetal lung
• Mode de croissance: Adherent
• Caryotype: 2n=46, diploid
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human foetal lung

Description de la lignée cellulaire

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

Application

It has been used as a cell substrate to study the production of recombinant MVA (modified vaccinia virus Ankara).

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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