Sf21 TitreHigh AC free

5030202, insect ovary, Spherical

• Code UNSPSC : 41106514

Propriétés

• product name: Sf21 TitreHigh AC free, 05030202
• Source biologique: insect ovary
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Spherical
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Spodoptera frugiperda pupal ovarian tissue, serum-free

Description de la lignée cellulaire

This cell line is a derivative of the Sf21 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf21 (Spodoptera frugiperda) insect cell line. The cell line Sf21 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf21 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.

Application

Protein expression using the Baculovirus vector system, serum-free

Milieu de culture

TiterHigh^™ Sf Insect Medium (Sigma I5408) no supplements.

Procédure de repiquage

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.

Autres remarques

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Informations légales

TiterHigh is a trademark of Sigma-Aldrich Co. LLC