C8374
Human Collagen Type IV
from human placenta, powder
Synonyme(s) :
Collagen from human placenta, Collagen Type 4
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.61
Produits recommandés
product name
Collagène from human placenta, Bornstein and Traub Type IV, powder
Source biologique
human placenta
Niveau de qualité
Pureté
>85% (SDS-PAGE)
Forme
powder
Technique(s)
cell culture | stem cell: suitable
Impuretés
HIV, hepatitis B and hepatitis C, none detected
Solubilité
0.5 M acetic acid: 1 mg/mL, clear to very slightly hazy
Température de stockage
−20°C
Informations sur le gène
human ... COL4A1(1282) , COL4A2(1284) , COL4A3(1285) , COL4A4(1286) , COL4A5(1287) , COL4A6(1288)
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Application
Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis. Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.
Actions biochimiques/physiologiques
During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.
Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.
Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.
Composants
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.
Attention
This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.
Notes préparatoires
This powder can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.
Remarque sur l'analyse
An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.
Autres remarques
Le collagène se classe en un certain nombre de types structurellement et génétiquement distincts. Nous utilisons la nomenclature proposée par Bornstein et Traub. Il est important de ne pas confondre les désignations utilisées par Sigma et les types de classification reconnus.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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