C6805

Monoclonal Anti-Collagen, Type VII antibody produced in mouse

clone LH7.2, ascites fluid

• Synonyme(s) : Anti-EBD1, Anti-EBDCT, Anti-EBR1, Anti-NDNC8
• Numéro MDL: MFCD00164570
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: LH7.2, monoclonal
• Contient: 15 mM sodium azide
• Espèces réactives: human, goat, marmoset, monkey, guinea pig, sheep, pig, bovine
• Technique(s): dot blot: suitable; immunocytochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 1:1,000 using human or other mammalian frozen sections; western blot: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: Q02388
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... COL7A1(1294)

Description

Description générale

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type VII is the crucial component of anchoring fibrils and mutation in type VII collagen gene (COL7A1) leads to epidermolysis bullosa dystrophica. Monoclonal anti-collagen, type VII antibody can be used to distinguish invasive melanoma from non-invasive melanoma by visualizing the appearance and integrity of epidermal basement membrane. It can also be used in immunoblotting. Mouse anti-collagen type VII antibody reacts specifically with an epitope located on collagenase digested type VII collagen (150 kDa). The product also reacts with the basement membrane zone of stratified squamous epithelia of sheep, pig, bovine, guinea pig, goat and human.

Collagen type VII alpha 1 chain (COL7A1) is majorly expressed in skin, secreted by keratinocytes and fibroblasts. The gene is located on human chromosome 3p21.31.

Immunogène

insoluble fractions of neonatal human foreskin epidermal cells

Application

Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in immuno-gold labelling.

Monoclonal anti-collagen type VII antibody can be used as a primary antibody in virus titration. It can also be used for the localization of type VII collagen in different immunochemical assays like dot blot, immunocytochemistry and ELISA.

Actions biochimiques/physiologiques

Collagen type VII alpha 1 chain (COL7A1) is required for epithelium-to-stroma anchorage in skin, mucosa and cornea. It is considered as an immediate-early response gene for transforming growth factor β (TGF-β)/SMAD (mothers against decapentaplegic) signaling pathway.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable