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C2522

Sigma-Aldrich

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥3,000 W-A units/mg protein

Synonyme(s) :

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II

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About This Item

Numéro CAS:
9001-03-0
Numéro de classification (Commission des enzymes):
4.2.1.1 (BRENDA, IUBMB)
Numéro CE :
232-576-6
Numéro MDL:
MFCD00081467
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bovine erythrocytes

Niveau de qualité

300

Forme

lyophilized powder

Activité spécifique

≥3,000 W-A units/mg protein

Poids mol.

30 kDa

pl 

~5.9

Température de stockage

−20°C

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Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Actions biochimiques/physiologiques

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.

Définition de l'unité

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Inhibiteur

Réf. du produit
Description
Tarif

C4911

Chlorothiazide, thiazide diuretic

A6011

Acetazolamide, ≥99%, powder

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Arne Schön et al.
Analytical biochemistry, 488, 45-50 (2015-08-05)
Protein aggregation is a major issue affecting the long-term stability of protein preparations. Proteins exist in equilibrium between the native and denatured or partially denatured conformations. Often denatured or partially denatured conformations are prone to aggregate because they expose to
Rui Hou et al.
ACS omega, 8(21), 19057-19071 (2023-06-05)
DNA-encoded library (DEL) is a powerful ligand discovery technology that has been widely adopted in the pharmaceutical industry. DEL selections are typically performed with a purified protein target immobilized on a matrix or in solution phase. Recently, DELs have also
Gérard Liger-Belair et al.
ACS omega, 8(25), 22844-22853 (2023-07-03)
When it comes to champagne tasting, dissolved CO2 is a key compound responsible for the very much sought-after effervescence in glasses. Nevertheless, the slow decrease of dissolved CO2 during prolonged aging of the most prestigious cuvees raises the issue of
Maria M Garcia-Alai et al.
Nature communications, 9(1), 328-328 (2018-01-25)
In clathrin-mediated endocytosis, adapter proteins assemble together with clathrin through interactions with specific lipids on the plasma membrane. However, the precise mechanism of adapter protein assembly at the cell membrane is still unknown. Here, we show that the membrane-proximal domains
Hajime Shigemitsu et al.
Nature communications, 11(1), 3859-3859 (2020-08-02)
Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases. Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies. Here, we describe a
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Protocoles

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

Objective: To standardize a procedure for the enzymatic assay of Carbonic Anhydrase (EC 4.2.1.1) for Wilbur-Anderson Units.

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