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B3804

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Synonyme(s) :

5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.83
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate

Niveau de qualité

100

Forme

liquid

Température de stockage

2-8°C

Catégories apparentées

Description générale

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Application

BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity[1][2] such as western blot analysis.[3]

Attention

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Autres remarques

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
MKCV4566
MKCT4192
MKCR6740
MKCR2607
MKCN6094

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Yu A Nashchekina et al.
Bulletin of experimental biology and medicine, 163(1), 123-128 (2017-06-06)
Collagen I gels with protein concentrations of 1, 2, and 3.5 mg/ml were prepared and embedded in a porous polylactide scaffold to reduce their contraction. Concentration of the gel did not affect its degradation. Collagen gels promoted the formation of
Hermann Ehrlich et al.
Nature chemistry, 2(12), 1084-1088 (2010-11-26)
The minerals involved in the formation of metazoan skeletons principally comprise glassy silica, calcium phosphate or carbonate. Because of their ancient heritage, glass sponges (Hexactinellida) may shed light on fundamental questions such as molecular evolution, the unique chemistry and formation
Stephen P Christiansen et al.
Investigative ophthalmology & visual science, 47(2), 605-613 (2006-01-25)
A common treatment for motility disorders of the extraocular muscles (EOMs) is a resection procedure in which there is surgical shortening of the muscle. This procedure results in rotation of the globe toward the resected muscle, increased resting tension across
Prafulla Katkar et al.
Metallomics : integrated biometal science, 6(1), 117-125 (2013-11-01)
The ability of oxindolimine copper(II) and zinc(II) complexes, known to have antitumor activity, to inhibit human topoisomerase IB has been tested through enzymatic kinetic assays and molecular docking simulations. These copper and zinc compounds are able to inhibit remarkably the
Stephen Hearty et al.
Journal of microbiological methods, 66(2), 294-312 (2006-02-07)
A panel of hybridomas was produced using intact Listeria monocytogenes serotype 1/2a cells as the immunogen. An IgG2a monoclonal antibody (mAb) 'mAb2B3' was isolated that reacted with L. monocytogenes but not with a representative panel of related Listeria spp. and
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Contenu apparenté

Antibody Support Reagents

Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.

Antibody Support Reagents

Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.

Antibody Support Reagents

Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.

Antibody Support Reagents

Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.

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