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A9171

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−AP

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About This Item

Numéro MDL:
MFCD00162223
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

200

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:35,000
dot blot: 1:100,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Immunoglobulin Y (IgY) is also known as egg yolk immunoglobulin and is found mainly in birds, amphibians and reptiles. IgY cannot activate mammalian complement system and cannot bind to mammalian rheumatoid factors, and protein A or G. IgY facilitates an essential role in veterinary science, functional food and bio-products. It also has a crucial role in controlling infectious diseases of bacterial and viral origin.

Spécificité

Anti-Chicken IgY (IgG) (whole molecule)-Alkaline Phosphatase antibody is specific for chicken IgY (IgG).

Immunogène

IgY (IgG) isolated from pooled normal chicken serum
IgY (IgG) isolated from pooled normal chicken serum.

Application

Anti-Chicken IgY (IgG) (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in:
  • immunoblotting
  • indirect enzyme linked immunosorbent assay (ELISA)
  • immunoglobulin assay
  • dot Blot
  • immunohistology

Actions biochimiques/physiologiques

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Yu-Chun Cai et al.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 27(6), 527-530 (2010-03-18)
Egg yolk immunoglobulin (IgY) is a major immunoglobulin from birds, amphibians and reptiles. IgY does not activate mammalian complement system, and does not bind to mammalian rheumatoid factors, protein A or G. In addition, it has a high yield, and
Paulina Escandon et al.
Veterinary medicine (Auckland, N.Z.), 10, 141-150 (2019-12-10)
This study evaluated the specificity of different avian secondary antibodies used in Western blot and dot-blot ELISA to detect avian bornavirus antibodies in bird plasma. Plasma samples were collected from: two Blue and gold macaws, one positive and one negative
M W C D Palliyeguru et al.
British poultry science, 52(3), 359-367 (2011-07-08)
1. The effect of three different levels of dietary trypsin inhibitor activity (achieved by varying the amount of non-toasted full fat soya bean in replacement for toasted full fat soya bean) on the incidence of spontaneously-occurring sub-clinical necrotic enteritis (NE)
Effect of trypsin inhibitor activity in soya bean on growth performance, protein digestibility and incidence of sub-clinical necrotic enteritis in broiler chicken flocks
Palliyeguru MW, et al.
British Poultry Science, 52(3), 359-367 (2011)
Ana Barbosa Marcondes de Mattos et al.
Frontiers in physiology, 14, 1252470-1252470 (2024-01-04)
Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease for which surgical or endovascular repair are the only currently available therapeutic strategies. The development of AAA involves the breakdown of elastic fibers (elastolysis), infiltration of inflammatory cells, and apoptosis of
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