A2909
Rabbit IgG−Agarose
saline suspension
Synonyme(s) :
IgG agarose beads
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
A propos de cet article
Conjugate:
agarose conjugate
Technique(s):
immunoprecipitation (IP): suitable
Application:
IP
Citations:
49
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderconjugate
agarose conjugate
Quality Segment
form
saline suspension
extent of labeling
≥5 mg per mL
technique(s)
immunoprecipitation (IP): suitable
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix spacer
1 atom
storage temp.
2-8°C
General description
IgGs are glycoprotein antibodies that modulate several immune responses. IgG-Agarose is an immunoadsorbent that can be used to purify antibodies, remove species specific cross-reacting antibodies, or remove contaminating antibodies from an antiserum preparation. Characteristically, cross-reacting antibodies may be removed from an antiserum preparation using an equal resin volume of IgG-Agarose. However, the resin to antiserum ratio will vary with individual applications. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively.
Application
Rabbit IgG antibody (20 μl/ml) crosslinked to agarose beads was used to isolate RAT tagged proteins from whole cell extracts of 293T cells.Rabbit IgG crosslinked to agarose beads were used for purification of tagged protein from mammalian whole cell extracts at 0.5 to 1.0mg total protein to 10 μl of beads.
Physical form
suspension in 0.5 M NaCl containing preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Classe de stockage
10 - Combustible liquids
Mot-clé
Warning
Codes de danger
Mentions de précaution
Hazard Classifications
Skin Sens. 1
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.