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A1960

Sigma-Aldrich

Aggrecan from bovine articular cartilage

lyophilized powder

Synonyme(s) :

Aggrecan Protein

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About This Item

Numéro MDL:
MFCD00676441
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

Source biologique

bovine articular cartilage

Niveau de qualité

200

Forme

lyophilized powder

Conditionnement

glass bottle of 1 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

salt, essentially free

Couleur

white

Solubilité

H2O: 2 mg/mL

Numéro d'accès UniProt

P13608

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

cow ... AGC1(280985)

Application

Aggrecan is a critical component for cartilage structure and the function of joints. Its synthesis and degradation are being studied for their roles in cartilage deterioration during joint injury disease and aging.

Actions biochimiques/physiologiques

This molecule produces a rigid, reversibly deformable gel that resists compression. It combines with hyaluronic acid to form very large macromolecular complexes that have an increased hydrohynamic volume and a significant increase (30-40%) in the relative viscosity of the solution.

Composants

Aggrecan is a major structural proteoglycan of the cartilage extracellular matrix. It is a large proteoglycan, with a molecular weight greater than 2,500 kDa composed of approximately 100-150 glycosaminoglycan chains attached to a core protein. The majority of these glycosaminoglycan chains are chondroitin/dermatan sulfate. Aggrecan contains three globular domains, G1, G2, and G3, which are involved in aggregation and hylauronan binding, cell adhesion and chondrycte apoptosis.

Attention

Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.

Notes préparatoires

This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

L Cao et al.
Experimental cell research, 246(2), 527-537 (1999-02-02)
A major feature of cartilage deterioration during joint injury and disease is aggrecan degradation and the loss of proteoglycan. Most of the degraded fragments are released into the circulatory system except the G1 domain which accumulates locally in the synovial
T E Hardingham et al.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies, 32(4), 249-257 (1994-04-01)
Aggrecan, the large aggregating proteoglycan from cartilage contains chondroitin sulphate and keratan sulphate attached to a multidomain protein core. It aggregates by binding to hyaluronan and this is further stabilised by a separate globular link protein. There are two structurally
N Ishiguro et al.
Arthritis and rheumatism, 42(1), 129-136 (1999-01-27)
To determine the relationship between matrix metalloproteinases (MMPs), their inhibitors, and the turnover of matrix molecules in articular cartilage from patients with osteoarthritis (OA). Synovial fluid samples were collected from the knees of 54 patients with OA. Radiographic evaluations and
H Hedlund et al.
The Journal of biological chemistry, 274(9), 5777-5781 (1999-02-20)
Aggrecan, the predominant large proteoglycan of cartilage, is a multidomain macromolecule with each domain contributing specific functional properties. One of the domains contains the majority of the keratan sulfate (KS) chain substituents and a protein segment with a proline-rich hexapeptide
C J Billington et al.
The Biochemical journal, 336 ( Pt 1), 207-212 (1998-11-10)
The breakdown of aggrecan in cartilage is, in part, mediated by an enzyme named aggrecanase that cleaves within the interglobular domain of the molecule between a glutamic residue and an alanine residue. Although the enzyme cleavage site has been identified
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