A1287

5-Azacytidine

Hybri-Max^™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Synonyme(s) : 4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-one, Ladakamycin
• Formule empirique (notation de Hill): C₈H₁₂N₄O₅
• Numéro CAS: 320-67-2
• Poids moléculaire : 244.20
• Numéro Beilstein : 620461
• Numéro CE : 206-280-2
• Numéro MDL: MFCD00006539
• Code UNSPSC : 12352207
• ID de substance PubChem : 24890525
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: synthetic (organic)
• Niveau de qualité: 200
• Qualité: Hybri-Max^™
• Stérilité: γ-irradiated
• Gamme de produits: BioXtra
• Forme: lyophilized powder
• Technique(s): cell culture | hybridoma: suitable
• Impuretés: endotoxin, tested
• Pf: 226-232 °C (dec.) (lit.)
• Solubilité: acetic acid: water (1:1): 5 mg/mL, clear, colorless
• Spectre d'activité de l'antibiotique: viruses
• Mode d’action: DNA synthesis | interferes
• Température de stockage: −20°C
• Chaîne SMILES: NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
• InChI: 1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
• Clé InChI: NMUSYJAQQFHJEW-KVTDHHQDSA-N
• Informations sur le gène: human ... DNMT1(1786) , DNMT3A(1788)

Description

Description générale

Chemical structure: nucleoside

Application

5-Azacytidine may be used to induce in vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. It may also be used to study the effects of 5-Azacytidine on the immune regulatory myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs).

Used to study the effects of the DNA incorporated molecule on DNA methylation processes.

Actions biochimiques/physiologiques

5-Azacytidine is a deoxycytidine analog and a demethylating agent. It acts as a potential antineoplastic agent for acute myelogenous leukemia. 5-Azacytidine activates repressed genes by inhibiting DNA methylation. 5-Azacytidine also affects protein synthesis by altering the RNA function and stability.

A potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase, an important regulatory mechanism of gene expression, gene activation and silencing.

Reconstitution

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine.

Informations légales

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302,H341,H350,H360FD,H372,H410
• Conseils de prudence: P202 - P260 - P264 - P273 - P301 + P312 - P308 + P313
• Classification des risques: Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges