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Principaux documents

83702

Sigma-Aldrich

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

Synonyme(s) :

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

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About This Item

Formule linéaire :
C21H17ClN2O3
Numéro CAS:
Poids moléculaire :
380.82
Beilstein:
6030951
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352116
ID de substance PubChem :
Nomenclature NACRES :
NA.32
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Qualité

for fluorescence

Niveau de qualité

Gamme de produits

BioReagent

Essai

≥85% (HPLC)

Forme

powder

Impuretés

≤10% water

Solubilité

ethanol: 20 mg/mL, clear, red

Adéquation

in accordance for fluorescence

Chaîne SMILES 

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

Clé InChI

MYFATKRONKHHQL-UHFFFAOYSA-N

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Description générale

Rhodamine 123 is a fluorescent dye.[1]

Application

Rhodamine 123 has been used:
  • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays[2]
  • to label the cells for estimating the P-gp expression by flow cytometry[1]
  • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)[3]
  • the detection of mitochondrial membrane potential (ΔΨm)[4]

Actions biochimiques/physiologiques

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.[1]

Autres remarques

Supravital mitochondrial-specific dye[5]

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells
Stebbins MJ, et al.
Methods, 101, 93-102 (2016)
Assessment of the chemotherapeutic potential of a new camptothecin derivative, ZBH-1205
Wu D, et al.
Archives of Biochemistry and Biophysics, 604, 74-85 (2016)
Evidence of apoptosis in Raillietina echinobothrida induced by methanolic extracts of three traditional medicinal plants of Northeast India
Giri BR, et al.
Experimental Parasitology, 134(4), 466-473 (2013)
Fumihiko Takeuchi et al.
PloS one, 10(7), e0132030-e0132030 (2015-07-07)
The Myxozoa are oligo-cellular parasites with alternate hosts--fish and annelid worms--and some myxozoan species harm farmed fish. The phylum Myxozoa, comprising 2,100 species, was difficult to position in the tree of life, due to its fast evolutionary rate. Recent phylogenomic
X Ronot et al.
Biology of the cell, 57(1), 1-7 (1986-01-01)
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of

Articles

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Questions

1–5 of 5 Questions  
  1. Which is the storage conditions recommend for this product?

    1 answer
    1. This product may be stored at room temperature.

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  2. What are the excitation and emission wavelengths for Rhodamine 123 (Product Nos. 83702 and R8004)?

    1 answer
    1. Using ethanol as the solvent, the excitation maximum is  511 nm and the emission maximum is 534 nm.  The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.

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  3. Why would one select Rhodamine 123 (Product Nos. 83702 and R8004) instead of other rhodamine compounds?

    1 answer
    1. Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.

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  4. What is the solubility of Rhodamine 123, (Product Nos. 83702 and R8004)?

    1 answer
    1. Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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