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Sigma-Aldrich

Atto 425-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Synonyme(s) :

Biotin-Atto 425

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About This Item

Formule empirique (notation de Hill):
C37H53N5O7S
Poids moléculaire :
711.91
Numéro MDL:
MFCD04974818
Code UNSPSC :
12352108
eCl@ss :
34058011
ID de substance PubChem :
57648315
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Pureté

≥95.0% (HPCE)

Forme

powder

Fabricant/nom de marque

ATTO-TEC GmbH

Fluorescence

λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Chaîne SMILES 

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c3cc2OC1=O

InChI

1S/C37H53N5O7S/c1-5-48-34(45)26-19-24-18-25-23(2)21-37(3,4)42(28(25)20-29(24)49-35(26)46)17-11-14-32(44)39-16-10-6-9-15-38-31(43)13-8-7-12-30-33-27(22-50-30)40-36(47)41-33/h18-20,23,27,30,33H,5-17,21-22H2,1-4H3,(H,38,43)(H,39,44)(H2,40,41,47)

Clé InChI

LPVYZZMUDCZDQO-UHFFFAOYSA-N

Description générale

Atto 425 is a new label with high quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 436 nm, emission maximum 484 nm). It is optimized for excitation with argon laser, and is characterized by high photostability. It does nearly not show cis-trans-isomerization, which limits brightness and reproducibility for many other dyes.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Informations légales

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution.
Praus, P., et al.
Spectroscopy: An International Journal, 24, 303-307 (2010)
Nermin Seda Kehr et al.
Nanoscale, 2(4), 601-605 (2010-07-21)
Microcontact printing (mCP) is used to immobilize dyes and peptides asymmetrically, by a "peptide coupling" reaction, on monolayers of zeolite L crystals in the contact area between the stamp and the surface of the monolayer. Chemically patterned surfaces of monolayers
Richard S Agnes et al.
Journal of the American Chemical Society, 132(17), 6075-6080 (2010-04-13)
A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 115(39), 11519-11524 (2011-08-26)
The fluorescence of the benzothiazole dye thioflavin T (ThT) is a well-known test for amyloid fibril formation. It has now become evident that ThT can also be used for structural investigations of amyloid fibrils and even for the treatment of
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