108319

Chlorhydrate de Trizma^®

EMPROVE^® EVOLVE

• Synonyme(s) : Chlorhydrate de trométhane, TRIS HCl, TRIS hydrochloride, Tris(hydroxyméthyl)aminométhane hydrochloride
• Formule linéaire : NH₂C(CH₂OH)₃ · HCl
• Numéro CAS: 1185-53-1
• Poids moléculaire : 157.60
• Numéro Beilstein : 3675235
• Numéro MDL: MFCD00012590
• Code UNSPSC : 12161700

Propriétés

• Source biologique: synthetic
• Niveau de qualité: 400
• Description: Manufactured under appropriate controls for use as a raw material in BioProcessing
• Gamme de produits: EMPROVE^® EVOLVE
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• Impuretés: Endotoxin, tested; Microbial, tested; Trace Metals, tested
• Plage de pH utile: 7.0-9.0
• pKa (25 °C): 8.1
• Adéquation: suitable for manufacturing use
• Activité étrangère: Cytotoxicity, contains (tested); DNase, contains (tested); NICKase, contains (tested); Protease, contains (tested); RNase, contains (tested)
• Chaîne SMILES: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• Clé InChI: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Description générale

Our SAFC^® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

As part of our EMPROVE^® Program, our raw materials are offered with EMPROVE^® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.

Autres remarques

Le pH de tous les tampons dépend de la température et de la concentration. Pour les tampons Tris, le pH augmente d'environ 0,03 unité par °C de diminution de la température et diminue de 0,03-0,05 unité par dilution d'un facteur 10. Pour les applications précises, utiliser un pH-mètre soigneusement calibré avec une électrode de verre au calomel.

Informations légales

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable