PPB006

Solution tampon phosphate

powder, pH 7.4, pHast Pack^™

• Synonyme(s) : PBS
• Numéro MDL: MFCD00131855
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.21

Propriétés

• product name: Solution tampon phosphate, pH 7.4, pHast Pack^™, powder
• Gamme de produits: pHast Pack^™
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry)
• Impuretés: <5—2 ppm Heavy metals (Lead)
• Couleur: white
• pH: 7.4
• Solubilité: soluble, clear, colorless
• Traces de cations: Fe: <10 ppm
• Activité étrangère: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• Température de stockage: room temp

Description

Description générale

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Application

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

Caractéristiques et avantages

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Conditionnement

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Stockage et stabilité

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations légales

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable