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E7889

Sigma-Aldrich

Acide éthylènediaminetétraacétique disodium salt solution

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Synonyme(s) :

Acide (éthylènedinitro)tétraacétique disodium salt, EDTA disodium salt, EDTA-Na2

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About This Item

Formule linéaire :
[-CH2N(CH2CO2Na)CH2CO2H]2
Numéro CAS:
139-33-3
Poids moléculaire :
336.21
Beilstein:
3822669
Numéro MDL:
MFCD00070672
Code UNSPSC :
12352107
ID de substance PubChem :
24894660
Nomenclature NACRES :
NA.31
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Qualité

for molecular biology

Niveau de qualité

300

Stérilité

0.2 μm filtered

Forme

liquid

Pertinence de la réaction

reagent type: chelator

Concentration

0.5 M in H2O

pH

7.5-8.5

Adéquation

suitable for molecular biology

Application(s)

microbiology

Activité étrangère

DNase, RNase, NICKase and protease, none detected

Chaîne SMILES 

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

Clé InChI

ZGTMUACCHSMWAC-UHFFFAOYSA-L

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Catégories apparentées

Description générale

L'acide éthylènediaminetétraacétique (EDTA) est un acide polyprotique. Les quatre groupements acide carboxylique et les deux groupements amine de l'EDTA, avec leurs doublets non liants d'électrons, sont capables de chélater le calcium et plusieurs autres ions métalliques.
Le sel disodique de l'acide éthylènediaminetétraacétique en solution est un agent de chélation adapté aux applications de biologie moléculaire.

Application

Le sel disodique de l'acide éthylènediaminetétraacétique en solution a été utilisé :
  • dans de l'acide Tris-HCl pour digérer de la protéinase-K afin de déterminer la quantité d'ADN présente dans un échantillon cornéen.
  • avec de la papaïne pour digérer des échantillons cornéens afin de quantifier la teneur en glycosaminoglycanes (GAG).
  • en prétraitement pour détacher des cellules épithéliales cultivées jusqu'à une confluence de 80 %.
  • pour préparer des solutions-mères de tampon de lyse.
  • dans la préparation d'un tampon de lyse à l'urée.

Caractéristiques et avantages

Qualité biologie moléculaire

Notes préparatoires

Préparé avec de l′eau purifiée à 18 mégaohms et de l′EDTA de qualité réactif de biologie moléculaire (E 5134)

Autres remarques

Pour de plus amples informations sur notre gamme de Produits biochimiques, merci de compléter ce formulaire.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H373

Conseils de prudence

P260 - P314 - P501

Classification des risques

STOT RE 2 Inhalation

Organes cibles

Respiratory Tract

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000403705
0000403705
BCCM6671
0000395957
0000391927

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Yi-Yuan Ke et al.
Foods (Basel, Switzerland), 10(1) (2021-01-21)
Isothiocyanates (ITCs) are important functional components of cruciferous vegetables. The principal isothiocyanate molecule in broccoli is sulforaphane (SFN), followed by erucin (ERN). They are sensitive to changes in temperature, especially high temperature environments where they are prone to degradation. The
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
Joshua B Black et al.
Cell reports, 33(9), 108460-108460 (2020-12-03)
Technologies to reprogram cell-type specification have revolutionized the fields of regenerative medicine and disease modeling. Currently, the selection of fate-determining factors for cell reprogramming applications is typically a laborious and low-throughput process. Therefore, we use high-throughput pooled CRISPR activation (CRISPRa)
Gary Reynolds et al.
Science (New York, N.Y.), 371(6527) (2021-01-23)
The skin confers biophysical and immunological protection through a complex cellular network established early in embryonic development. We profiled the transcriptomes of more than 500,000 single cells from developing human fetal skin, healthy adult skin, and adult skin with atopic
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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Protocoles

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Questions

1–8 of 8 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How many divalent cations does Product E7889, EDTA disodium salt, complex with?

    1 answer

    1. EDTA forms 1:1 complex with divalent cations.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Can a solution of EDTA disodium salt, Product E7889, be autoclaved?

    1 answer

    1. Yes, solutions of EDTA may be autoclaved.

      Helpful?

  6. What is the pH of E7889, EDTA disodium salt solution?

    1 answer

    1. E7889 has a pH specification of 7.5 to 8.5 at 25 °C.

      Helpful?

  7. At what concentration should I use Product E7889, EDTA disodium salt, as an anticoagulant?

    1 answer

    1. The optimal concentration of EDTA to be used as an anticoagulant is 1.5 mg of EDTA disodium salt per ml of blood or 1-2 drops of a 2% EDTA solution per ml of blood.

      Helpful?

  8. What type of biological activity does Product E7889, EDTA disodium salt, exhibit?

    1 answer

    1. EDTA is a metal ion-dependent enzyme inhibitor. It may be used as a metalloprotease inhibitor at a concentration of 1-10 μm, or as a DNAse I inhibitor at a concentration of 1-10 mM.

      Helpful?

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