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B8647

Sigma-Aldrich

Brilliant Blue R Concentrate

methanol solution

Synonyme(s) :

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Formule empirique (notation de Hill):
C45H44N3NaO7S2
Numéro CAS:
Poids moléculaire :
825.97
Numéro C.I. (Colour Index):
42660
Numéro Beilstein :
5718025
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.47

product name

Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution

Forme

methanol solution

Niveau de qualité

Couleur

dark blue

Adéquation

suitable for SDS-PAGE

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

Clé InChI

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).
For detection of protein bands following electrophoresis.

Reconstitution

After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.

Informations légales

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Point d'éclair (°F)

57.2 °F - closed cup

Point d'éclair (°C)

14 °C - closed cup

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Crystallization of soluble proteins in vapor diffusion for x-ray crystallography.
Benvenuti M and Mangani S
Nature Protocols, 2, 1633-1651 (2007)
Overexpression of human apolipoprotein AI in transgenic rats and the hyperlipoproteinemia associated with experimental nephrosis.
Burkey BF, et al.
Journal of Lipid Research, 36.7, 1463-1473 (1995)
The effect of ethanol or hepatotoxin exposure on rat transferrin desialylation.
Lof K, et al.
Alcohol and Alcoholism (Oxford, Oxfordshire), 31, 445-451 (1996)
Katharina Stock et al.
Cancer cell international, 19, 77-77 (2019-04-13)
The nucleation-promoting factor cortactin is expressed and promotes tumor progression and metastasis in various cancers. However, little is known about the biological role of cortactin in the progression of pancreatic ductal adenocarcinoma (PDAC). Cortactin and phosphorylated cortactin (Y421) were investigated
G Houen et al.
Electrophoresis, 18(5), 701-705 (1997-05-01)
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of

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