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252549

Sigma-Aldrich

Formaldéhyde solution

Formaldehyde solution

ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

Synonyme(s) :

Formol

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About This Item

Formule linéaire :
HCHO
Numéro CAS:
50-00-0
Poids moléculaire :
30.03
Beilstein:
1209228
Numéro MDL:
MFCD00003274
Code UNSPSC :
12352114
ID de substance PubChem :
24855218
Nomenclature NACRES :
NA.21
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Qualité

ACS reagent

Niveau de qualité

200

Densité de vapeur

1.03 (vs air)

Pression de vapeur

52 mmHg ( 37 °C)
52 mmHg ( 37 °C)

Forme

liquid

Température d'inflammation spontanée

572 °F

Contient

10-15% Methanol as stabilizer (to prevent polymerization)

Concentration

36.5-38.0%
37 wt. % in H2O

Impuretés

≤0.006 meq/g Titr. acid

Résidus de calcination

≤0.005%

Couleur

APHA: ≤10

Indice de réfraction

n20/D 1.3765

Solubilité

water: soluble

Densité

1.09 g/mL at 25 °C (lit.)

Traces d'anions

chloride (Cl-): ≤5 ppm
sulfate (SO42-): ≤0.002%

Traces de cations

Fe: ≤5 ppm
heavy metals: ≤5 ppm (by ICP-OES)

Adéquation

suitable for manufacturing use (Laboratory chemicals)

Chaîne SMILES 

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

Clé InChI

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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Description générale

Le formaldéhyde (formol) est obtenu par oxydation du méthanol. Ce produit est composé de 37 % de formaldéhyde et d′impuretés telles que le méthanol, de petites quantités d′acide formique, d′aldéhydes et de cétones. Il est utilisé comme agent dénaturant dans l′électrophorèse sur gel d′agarose d′ARN en présence de formaldéhyde.[1][2]

Application

Le formaldéhyde en solution a été utilisé comme agent de fixation des cellules lors de l′imagerie par immunofluorescence et pour la réticulation des cellules dans l′essai d′immunoprécipitation de la chromatine (ChIP).[3]
Le formaldéhyde en solution a été utilisé pour le couplage ou la fixation de cellules dans le test d'immunoprécipitation de la chromatine (ChIP).[4][5] Le formaldéhyde en solution a été utilisé pour la fixation de cellules à des fins d'imagerie.[6][7]

Forme physique

Ce produit est une solution à environ 37 % (par poids) de gaz de formaldéhyde dans de l′eau.

Souvent commandé avec ce produit

Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

Organes cibles

Eyes,Central nervous system, Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

132.8 °F - closed cup

Point d'éclair (°C)

56 °C - closed cup

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Certificats d'analyse (COA)

Lot/Batch Number
MKCW1343
MKCV9749
MKCX0729
MKCV9260
MKCW4227

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Intracellular protein and nucleic acid measured in eight cell types using deep-ultraviolet mass mapping.
Cheung MC et al.
Cytometry, 83, 540-540 (2013)
Slug contributes to cancer progression by direct regulation of ERa signaling pathway.
Li Y et al.
International Journal of Oncology, 46, 1461-1461 (2015)
Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.
Cole NB et al.
Experimental Cell Research, 314, 2076-2076 (2008)
SIRT6 protects against endothelial dysfunction and atherosclerosis in mice.
Xu S et al.
Aging (Albany. NY.), 8, 1064-1064 (2016)
Farrell RE
RNA Methodologies: A Laboratory Guide for Isolation and Characterization (2010)
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Questions

1–10 of 16 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Can you store formaldehyde in plastic?

    1 answer

    1. This product is packaged in glass bottles. Generally, formaldehyde solution can be used in plastic containers. However, it is advised that the unused material be preserved in its original packaging.

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  4. What type of packaging for different packages?

    1 answer

    1. This product is packaged in amber glass bottles with polypropylene caps with HDPE liners. The bottle sizes are as follows:
      252549-25 ML in 50 ml bottles
      252549-100 ML in 100 ml bottles
      252549-500ML in 500 ml bottles
      252549-1L in 1L bottles
      252549- 4L in 4L bottles

      Helpful?

  5. Can formaldehyde solution 37% be diluted to 10% formalin for fixing cells for staining? Do you dilute it with around 2.7 part water? Thank you!

    1 answer

    1. How much water should be added to 100mL of a 37% solution to reduce its strength to a 10% solution?
      Use C1 x V1= C2 x V2
      37% x 100ml = 10% x V2
      Therefore V2 = (37% x 100ml) / 10%
      = 370 mL

      Subtract the total original volume from the new volume.
      370 mL - 100 mL = 270 mL
      So to reduce 100mL of 37% to 10% solution, add 270 mL of water to 100 mL of Formaldehyde solution - 252549-25ML.

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  6. Which formaldehyde solution is recommended for use with the HT102A Reticulum kit?

    1 answer

    1. When a silver stain procedure recommends a 10% formalin solution, it is referring to a 10% aqueous formalin solution, which is prepared by diluting 1 part of 37% to 40% formaldehyde with 9 parts deionized water. For histology, it is advisable to use ACS reagents when the product meets ACS Specifications. Suitable products for this purpose would be product numbers 252549 or FX0410. If a 37% to 40% formaldehyde solution is available, it should work; however, it's important to avoid using old bottles of this solution that have been in the lab for several years, as impurities can form over time. Solutions used in histological procedures should typically be replaced every 2 years to ensure effectiveness.

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  7. Is the methanol content in the CoA expressed as volume percent or weight percent?

    1 answer

    1. The methanol content is expressed as weight percent.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  9. Is the concentration of Product No. 252549, Formaldehyde solution, expressed as a weight percent solution?

    1 answer

    1. Yes, 37% is a reference to weight percent solution.  Unless otherwise noted, this is the standard practice for reporting concentration by percent. In terms of volume percent, this solution would be approximately 40% by volume.

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  10. What is the difference between formaldehyde and paraformaldehyde?

    1 answer

    1. Paraformaldehyde is the polymeric form of formaldehyde. Paraformaldehyde is a solid, and formaldehyde is a gas. Formaldehyde solutions consist of formaldehyde gas dissolved in a solvent of some kind.

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1–10 of 16 Questions  

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