198161

Toluidine Blue O

certified by the Biological Stain Commission

• Synonyme(s) : Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
• Formule empirique (notation de Hill): C₁₅H₁₆ClN₃S
• Numéro CAS: 92-31-9
• Poids moléculaire : 305.83
• Numéro C.I. (Colour Index): 52040
• Numéro CE : 202-146-2
• Numéro MDL: MFCD00011934
• Code UNSPSC : 12171500
• ID de substance PubChem : 24851865
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: certified by the Biological Stain Commission
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, 80%
• Couleur: dark green
• λmax: 626 nm
• ε (coefficient d'extinction): ≥13000 at 241-247 nm in H₂O; ≥28000 at 287-293 nm in H₂O; ≥40000 at 625-635 nm in H₂O
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
• InChI: 1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
• Clé InChI: GEDVVYWLPUPJJZ-UHFFFAOYSA-N

Description

Description générale

A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Actions biochimiques/physiologiques

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Adéquation

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)