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10109134001

Roche

RNase

from bovine pancreas

Synonyme(s) :

Rnase

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About This Item

Code UNSPSC :
12352204

Source biologique

bovine pancreas

Niveau de qualité

100

Forme

dry powder (prepared from crystallized ribonuclease)

Activité spécifique

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

Conditionnement

pkg of 500 mg

Fabricant/nom de marque

Roche

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Spécificité

Heat inactivation: 1 mM EDTA

Application

RNase has been used in DNA ladder assay.
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.

Définition de l'unité

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Notes préparatoires

Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

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Ribonucleases (2011)
S Saito et al.
The Journal of biological chemistry, 275(44), 34528-34533 (2000-08-10)
Apoptosis of cardiac myocytes is one of the causes of heart failure. Here we examine the mechanism by which the activation of beta-adrenergic receptor induces cardiomyocyte apoptosis. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling and DNA ladder analyses revealed that
Ognjen Sekulović et al.
Methods in molecular biology (Clifton, N.J.), 1476, 143-165 (2016-08-11)
Bacteriophages (phages) are present in almost, if not all ecosystems. Some of these bacterial viruses are present as latent "prophages," either integrated within the chromosome of their host, or as episomal DNAs. Since prophages are ubiquitous throughout the bacterial world
Mihnea R Mangalea et al.
Cell host & microbe, 29(5), 726-739 (2021-05-07)
Rheumatoid arthritis (RA) is an autoimmune disease characterized in seropositive individuals by the presence of anti-cyclic citrullinated protein (CCP) antibodies. RA is linked to the intestinal microbiota, yet the association of microbes with CCP serology and their contribution to RA
David Olmeda et al.
Molecular biology of the cell, 14(7), 2844-2860 (2003-07-15)
Beta-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. Beta-catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems beta-catenin also acts as
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