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Sigma-Aldrich

YUMM2.1 Mouse Melanoma Cell Line

Mouse

Synonyme(s) :

Yale University Mouse Melanoma 2.1 cell line, Yale University Mouse Melanoma cell line

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81

product name

YUMM2.1 Mouse Melanoma Cell Line,

Source biologique

mouse

Niveau de qualité

Conditionnement

vial of ≥1X10⁶ cells/vial  vials

Fabricant/nom de marque

Millipore

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

liquid nitrogen

Température de stockage

−196°C

Description générale

The YUMM2.1 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing. The YUMM2.1 cell line harbors the Braf V600E mutation and is homozygous negative for wild-type Pten, heterozygous for Cdkn2, and characterized by expression of stable beta-catenin via partial Cre/lox recombination.

Application

  • Each vial contains > 1X106 viable cells.
  • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
  • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
  • Cells are negative for mycoplasma contamination.

Caractéristiques et avantages

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2 harbors the Braf V600E mutation and is homozygous negative for wt Pten and is Cdkn2+.

Description de la cible

The promise of immune-based therapies in cancer and increasingly successful application of these approaches have emphasized the necessity of immune-competent models to evaluate immunological responses to cancer cells. Immunocompetent genetically-engineered mouse models with distinct genetic drivers of melanoma are essential for identifying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgroundsYUMM2.1 cells are syngenic with the immunocompetent C57BL/6 mouse background and retain genetic markers of the Braf/Pten mouse model, characterized by activation of Braf and inactivation of Pten and Cdkn2a. The YUMM2.1 cell line harbors a stabilized beta-catenin allele from excision of exon 3, a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis, enhances metastasis, and controls tumor differentiation. Exon 3 excision is analogous to human beta-catenin stabilizing mutations found in human melanomas.Mutation: BrafV600E/wt Pten−/− Cdkn2+/−Bcat loxex3/wt. Partial Bcat STA/wt recombination​. Male.References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

Stockage et stabilité

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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