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QIA127

Sigma-Aldrich

Rapid Cell Proliferation Kit

Synonyme(s) :

Proliferation assay kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 500 tests

Niveau de qualité

100

Conditionnement

pkg of 1 96-well plate(s)

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

Entrée

sample type cultured cells (1,000-50,000 cells)

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

Caractéristiques et avantages

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

Composants

WST-1, 1-Methoxy PMS, and a user protocol.

Avertissement

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Caractéristiques

Assay Time: 0.5 - 4 h

Principe

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

Notes préparatoires

• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

Autres remarques

Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

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Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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