MABS2157
Anti-CH25H Antibody, clone 18G9
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
18G9, monoclonal
Poids mol.
calculated mol wt 31.75 kDa
observed mol wt ~34 kDa
Produit purifié par
using protein G
Espèces réactives
human, mouse
Conditionnement
antibody small pack of 100 μg
Technique(s)
ELISA: suitable
western blot: suitable
Isotype
IgG1κ
Séquence de l'épitope
Unknown
Numéro d'accès Protein ID
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CH25H(9023)
Description générale
Cholesterol 25-hydroxylase (UniProt: O95992; also known as EC:1.14.99.38, Cholesterol 25-monooxygenase, h25OH) is encoded by the CH25H gene (Gene ID: 9023) in human. CH25H is a multi-pass membrane glycoprotein with three transmembrane domains (aa 38-58; 84-104; and 121-141) that catalyzes the formation of 25-hydroxycholesterol from cholesterol, which in turn leads to repression of cholesterol biosynthesis. It is also reported to play a role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H is an interferon inducible enzyme that has broad-spectrum antiviral functions. Stimulation of macrophage Toll-like receptors (TLR) is also reported to increase its expression and synthesis of 25-hydroxycholesterol. Treatment of naïve B cells with nanomolar concentrations of 25-hydroxycholesterol is shown to suppress IL-2-mediated stimulation of B cell proliferation, repress activation induced cytidine deaminase expression, and reduce IgA production. Deletion of CH25H gene in mouse is shown to increase in serum IgA. In testis tissue, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. (Ref.: Bauman, DR., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(39); 16764-16769).
Spécificité
Clone 18G9 is a mouse monoclonal antibody that detects Cholesterol 25-hydroxylae (CH25H).
Immunogène
His-tagged full length recombinant human Cholesterol 25-hydroxylase.
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cholesterol 25-hydroxylase (CH25H) in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).
Tested Applicaitons
Western Blotting Analysis: A representative lot detected CH25H in Western Blotting applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9; Tuong, Z.K., et. al. (2016). PLoS One. 11(1):e0147179).
ELISA Analysis: A representative lot detected CH25H in ELISA applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cholesterol 25-hydroxylase (CH25H) in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).
Tested Applicaitons
Western Blotting Analysis: A representative lot detected CH25H in Western Blotting applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9; Tuong, Z.K., et. al. (2016). PLoS One. 11(1):e0147179).
ELISA Analysis: A representative lot detected CH25H in ELISA applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CH25H, clone 18G9, Cat. No. MABS2157, is a mouse monoclonal antibody that detects Cholesterol 25-hydroxylase (CH25H) and is tested for use in ELISA and Western Blotting.
Forme physique
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Stockage et stabilité
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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