MABF235

Anti-AIRE Antibody, clone Mab155

clone Mab155, 1 mg/mL, from mouse

• Synonyme(s) : Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, APECED protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: Mab155, monoclonal
• Espèces réactives: human
• Concentration: 1 mg/mL
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_000374
• Numéro d'accès UniProt: O43918
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AIRE(326)

Description

Description générale

AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).

Immunogène

His-tagged recombinant protein corresponding to human AIRE.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

This Anti-AIRE Antibody, clone Mab155 is validated for use in Flow Cytometry and Western Blotting and Immunocytochemistry for the detection of AIRE.

Western Blotting Analysis: A representative lot detected AIRE in Cos1 transfected cell lysate (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Immunocytochemistry Analysis: A representative lot detected AIRE in freshly isolated endogenous peripheral blood lymphocytes (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).

Qualité

Evaluated by Flow Cytometry in human PBMC cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected AIRE in human PBMC cells.

Description de la cible

58 kDa calculated

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable