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Key Documents

MAB4417

Sigma-Aldrich

Anti-Achaete Scute homolog 2 Antibody, clone 8F1

clone 8F1, from mouse

Synonyme(s) :

achaete-scute complex (Drosophila) homolog-like 2, achaete-scute complex homolog 2 (Drosophila), achaete-scute complex homolog-like 2, achaete-scute complex-like 2, achaete-scute complex-like 2 (Drosophila), mammalian achaete/scute homologue 2

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

8F1, monoclonal

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

mouse (based on 100% sequence homology)

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NP_032580.2

Numéro d'accès UniProt

O35885

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ASCL2(430)

Description générale

Achaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).

Spécificité

This antibody recognizes Achaete Scute homolog 2.

Immunogène

His-tagged recombinant protein corresponding to mouse Achaete Scute homolog 2.

Application

Anti-Achaete Scute homolog 2 Antibody, clone 8F1 is an antibody against Achaete Scute homolog 2 for use in WB, IH.
Immunohistochemistry: A previous lot of this antibody was used in immunocytochemistry by an independent laboratory (Van der Flier, et al., 2009).

Qualité

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 on 10 µg of human placenta tissue lysate.

Description de la cible

~ 20 kDa

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Qingyu Wang et al.
Stem cell reports, 15(2), 374-388 (2020-07-11)
Intestinal regeneration is crucial for functional restoration after injury, and nutritional molecules can play an important role in this process. Here, we found that arachidonic acid (AA) serves as a direct proliferation promoter of intestinal epithelial cells that facilitates small
Jie Wang et al.
Oncotarget, 9(34), 23320-23333 (2018-05-29)
Achaete-scute-like 2 (ASCL2) is a transcription factor containing a basic helix-loop-helix (bHLH) domain and is a downstream target of Wnt signaling in intestinal stem cells. Bufalin is the primary active ingredient in Chan Su, a traditional Chinese medicine obtained from
Rosemary Oh-McGinnis et al.
Developmental biology, 351(2), 277-286 (2011-01-18)
Several imprinted genes have been implicated in the regulation of placental function and embryonic growth. On distal mouse chromosome 7, two clusters of imprinted genes, each regulated by its own imprinting center (IC), are separated by a poorly characterized region
Justine Creff et al.
Cell reports, 42(6), 112659-112659 (2023-06-16)
p57Kip2 is a cyclin/CDK inhibitor and a negative regulator of cell proliferation. Here, we report that p57 regulates intestinal stem cell (ISC) fate and proliferation in a CDK-independent manner during intestinal development. In the absence of p57, intestinal crypts exhibit
Kevin C Allan et al.
Cell stem cell, 28(2), 257-272 (2020-10-23)
Mammalian cells respond to insufficient oxygen through transcriptional regulators called hypoxia-inducible factors (HIFs). Although transiently protective, prolonged HIF activity drives distinct pathological responses in different tissues. Using a model of chronic HIF1a accumulation in pluripotent-stem-cell-derived oligodendrocyte progenitors (OPCs), we demonstrate
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