MAB4385
Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85
clone TRA-1-85, Chemicon®, from mouse
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
TRA-1-85, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Entrée
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
Isotype
IgG1
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Description générale
The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.
Spécificité
This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.
SPECIES REACTIVITIES:
Refer to comments regarding specificity
SPECIES REACTIVITIES:
Refer to comments regarding specificity
Immunogène
2102Ep human Embryonal Carcinoma Cells
Epitope: blood group antigen Ok(a)
Application
Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.
FACS Analysis: a starting range of 5-20 μg/ml is suggested.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Description de la cible
35-69 kDa
Forme physique
Format: Purified
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide
Stockage et stabilité
Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months
Remarque sur l'analyse
Control
Hemopoietic cells and a wide range of normal human tissues
Hemopoietic cells and a wide range of normal human tissues
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Jee Myung Yang et al.
Experimental & molecular medicine, 53(4), 631-642 (2021-04-09)
Retinal degenerative disorders, including age-related macular degeneration and retinitis pigmentosa (RP), are characterized by the irreversible loss of photoreceptor cells and retinal pigment epithelial (RPE) cells; however, the long-term effect of implanting both human induced pluripotent stem cell (hiPSC)-derived RPE
Linda Harkness et al.
Methods in molecular biology (Clifton, N.J.), 873, 33-51 (2012-04-25)
In 1998, a development occurred in stem cell biology with the first report of the derivation of a human embryonic stem cell (hESC) line. Since then a number of techniques have been used to derive and characterise hESCs. Here, we
Budd A Tucker et al.
eLife, 2, e00824-e00824 (2013-08-31)
Next-generation and Sanger sequencing were combined to identify disease-causing USH2A mutations in an adult patient with autosomal recessive RP. Induced pluripotent stem cells (iPSCs), generated from the patient's keratinocytes, were differentiated into multi-layer eyecup-like structures with features of human retinal
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